Research Journal of Agriculture and Biological Sciences, 5(2): 161-166, 2009
© 2009, INSInet Publication
Static Magnetic Field Influence on Elements Composition in Date Palm
(Phoenix dactylifera L.)
1Faten Dhawi, 2Jameel M. Al-Khayri and 3Essam Hassan
1Department of Botany and Microorganism, Girls Science College, King Faisal University, Dammam 31113, Saudi Arabia.
2Date Palm Research Center; Department of Agricultural Biotechnology, College of Agricultural
and Food Sciences, King Faisal University, P.O. BOX 420, Al-Hassa 31982, Saudi Arabia.
3Electrical Engineering Department, King Fahd University of Petroleum and Minerals, Dhahran 31261, Saudi Arabia.
Abstract: Living cells possess electric charges exerted by ions or free radicals, which act as endogenous magnets. These endogenous magnets can be affected by exogenous magnetic field, which can orient unpaired electrons. Treatments with magnetic field are assumed to enhance seed vigor by influencing the biochemical processes that involve free radicals, and by stimulating activity of proteins and enzymes. Numerous studies suggested that magnetic field increases ions uptake and consequently improves nutrition value which could be a good alternative for chemical treatments. Seedlings of date palm (Phoenix dactylifera L.) were treated with varying doses of static magnetic field (SMF) in order to evaluate the effect on elements uptake. The SMF source is a magnetic circuit set to produce three levels of magnetic field intensities (10, 50 and 100 mT). Seedlings were exposed to these magnetic fields for different periods: 0, 30, 60, 180, 240 and 360 min. Leaf samples were subjected to chemical analysis for elements (Mg, Ca, Na, P, K, Fe, Mn and Zn) using inductive couple plasma (ICP) spectroscopy. The results revealed that concentrations of Ca, Mg, Mn, Fe, Na, K, and Zn increased, while P concentration decreased with raising SMF intensities and durations of exposure. Static magnetic field has a potential to enhance growth due to the positive effect on the plant major elements such as Ca and Mg, but negative electrical charges on the plants inhibited the uptake of anions such as P. Increasing ions may elevate the nutrition value of date palm plants.
K ey w ords:
The exact mechanism of the effect of static magnetic field (SMF) on living organisms is still unclear. Plants cells affected by magnetic field can response in unpredictable way according to many factors including species, intensity of magnetic field (MF)and exposure period[1,2]. It has been reported that external magnetic fields influence both the activation of ions and polarization of dipoles in living cells. Response of the cells under time varying magnetic fields is contingent not only on the wavelength and amplitude but also on how well the exogenous MF matched the phase of the cell’s own oscillators; matched versus unmatched phase gives opposite results.
The forces induced by magnetic fields may be large enough to affect any process that can change the rate of movement of electrons significantly. Studies on the meristematic cells of plants have shown that MF effects normal metabolisms and has impact on cellular
division. An optimal external electromagnetic field could accelerate the activation of plant growth, especially seed germination[7,8,9,10].
Nutrition value could be enhanced by MF treatment. Sharaf El-Deen. noticed that MF increased amino acids, Ca and K content in mushroom (Agaricus bispours). In addition, magnetic field pretreatment of seeds was reported to increased lipid oxidation and ascorbic acid contents in cucumber (Cucumis sativus) . the sugar content in sugar beet roots (Beta vulgaris) and gluten in wheat (Triticum aestivum)[13, 14]. Magnetic field may play an important role in cation uptake capacity and has a positive effect on immobile plant nutrient uptake[15 ]. Therefore, MF could be a substitution of chemical additives, which can reduce toxins in raw materials and thus raise the food safety. There were few studies linking magnetic field with elements accumulation in plants of strawberry (Fragaria x ananassa). and wheat. However, literatures related to the effect of magnetic field on
Corresponding Author: Faten Dhawi, Department of Botany and Microorganism, Girls Science College, King Faisal University, Dammam 31113, Saudi Arabia.
Emal: [email protected],
ions accumulation in date palm were not encountered. The objective of this study was to evaluate ions accumulation in date palm in response to various intensities and durations of static magnetic field.
MATERIALS AND METHODS
Plant material: Seeds of date palm (cv. Khalas) were sterilized with 1% sodium hypochlorite for 5 min, soaked in water for 24 h at 37°C then germinated over moist filter paper at temperature of 37°C. At age of 15 days, seedlings were placed in 9 cm Petri dishes and subjected to SMF treatment.
Exposure to static magnetic field: The SMF was applied at Electrical Engineering Department in King Fahd University of Petroleum and Minerals (KFUPM) using, a static magnetic circuit with induction at three
intensity at three levels (10, 50 and 100 mT) and exposure duration at 6 levels including control samples (0, 30, 60, 180, 240 and 360 min).
Data were subjected to analysis of variance (ANOVA). Least significant difference (LSD) test applied to compare the elements results of the groups exposed to magnetic field with the control. For the statistical evaluation of the results, significance was defined by a probability level of p<0.05.
RESULTS AND DISCUSSION
The current study shows that elements composition are significantly affected by the intensity of the SMF and the duration of exposure as indicated by the significant two-way interaction based on ANOVA (Table 1). Major elements were affected by SMF intensities and duration (Figure1 A, B, C). Amount of
levels (10, 50 and 100 mT) for 0, 30, 60, 180, 240,
Ca and Mg increased significantly; while P+
and 360 min. The magnetic circuit consisted of two coils each of 480 turns per coil wound on carbon steel and loaded by variable currents to achieve variable magnetic field intensities. The pole pieces cross section is made with 10 cm internal diameter to enable placing the 9 cm petri dish horizontally.
decreased significantly; this trend grew gradually from 10 mT to 100 mT treatments.
Minor elements were also affected by SMF and increased significantly also; Mn, Fe and Zn average increased with increasing dose 10- 100 mT and durations (30- 360 min) (Figure2 A, B, C). Potassium
After treatment, each seedling was planted in 20-
and sodium (Na) pump were also affected
cm plastic pots containing potting mix (1 soil: 1 peat moss: 1 vermiculate) and maintained in greenhouse under natural light at temperature of 30°C – 41°C with 50% of relative humidity.
Analyzing and measuring elements: Elements were measured using Inductive Couple Plasma Spectroscopy
-Optical Emission Spectrometry (ICP-OES) (Varian- liberty- 730-ES simultaneous ICP-OES series II, USA). Leaves were oven dried at 70ºC for 24 h using (Duo- Vac oven Lab Line, 3620 Vacuum Oven, USA). Microwave-assisted digestion system was used to extract elements from leaf samples in a closed microwave system[17,18]. Dried sample digested by accurately weighing 0.25 g of sample into Teflon® PFA lined microwave digestion vessels and adding 3 ml of 10 M HNO3 (Merck Tracepur) and 1 ml of H2O. Microwave digestion applied at power of 600 W and pressure of 350 PSI, in two stages: first, at 120 ºC for
3 min and second at 200 ºC for 10 min. Following digestion, the solutions were allowed to cool, transferred to 25 ml volumetric flasks, and diluted to volume with >18 cm3 deionized water.
Experimental design and Statistical analysis: The experiment was designed with 7 replications per treatment. Total of 126 seedlings were used for this experiment. The experiment was setup as a factorial design 3×6. Two factors were involving magnetic field
significantly by SMF 10 -100 mT (Figure 3 A, B, C).
Discussion: The earth magnetic field influences the movement and absorption of elements. Liboff. suggested that magnetic fields can interact in a resonant manner with endogenous AC electric fields in biological systems. Static magnetic fields have been reported to affect the diffusion of biological particles in solutions by inducing Lorentz force or Maxwell stress. Lorentz force would influence the diffusion of charged particles such as various ions including plasma proteins . The orientation of ferromagnetic particles and the modulation of radical-pair reactions have been proposed as mechanisms for the observed effects of MF. Magnetic treatments are assumed to enhance plants seed vigor by influencing the biochemical processes that involve free radicals, and by stimulating the activity of proteins and enzymes[23,24]. have emphasized that 50-60 Hz and 10-100 mT magnetic fields has caused some changes on the permeability of plasma membrane at Vicia faba tip cell.
A study on tomato plants showed that the application of MF to irrigation water increased nutrient element contents of plants[25 ]. Ions up take increased flowing MF treatment, Marschner. suggested that owing to plant cells having negative electrical charge, they take up ions with a positive electrical charge.
T able 1: A n a lys is o f v a rian c e o f e lem e n ts a c c u m u lation u n d e r s tatic m a g n e tic field imp a c t. Factor df MS F p
C alcium Intensity 2 20045905.3 864.443314 0.0001
Time 5 11234585.2 484.471113 0.0001
———————————————————————————————————————————————————————————- Intensity X Time 10 1046908.12 45.146 0.0001
———————————————————————————————————————————————————————————- Error 108 23189.381
M agnes ium Intensity 2 1320459 112.53 0.0001
Time 5 464099 39.55 0.0001
Intensity X Time 10 56715 4.83 0.0001
Error 108 11734
M ang anese Intensity 2 4281.7 529.78 0.0001
Time (min) 5 2465.8 305.10 0.0001
Phosphorus Intensity 2 3095806.1 235.36 0.0001
Time 5 2885509 219.4 0.0001
Intensity X Time 10 144650.3 10.99 0.0001
———————————————————————————————————————————————————————————- Error 108 13153.18
———————————————————————————————————————————————————————————- Intensity 2 1055673 77.4 0.0001
———————————————————————————————————————————————————————————- Time 5 479076.508 35 0.0001
Intensity X Time 10 112829.4 8.3 0.0001
———————————————————————————————————————————————————————————- Error 108 13642.6
Sodium Intensity 2 7534921 1036.04 0.0001
Time (min) 5 1914055 263.18 0.0001
Intensity X Time 10 379501 52.18 0.0001
Error 108 7273
Iron Intensity 2 2478.5 342.05 0.0001
Time 5 1240.8 171.24 0.0001
Intensity X Time 10 179.9 24.82 0.0001
Error 108 7.2
Zinc Intensity 2 950.2 143.9 0.0001
Time 5 637.9 96.61 0.0001
Intensity X Time 10 93.6 14.18 0.0001
Error 108 6.6
Fig. 1: Macro elements accumulation affected by static magnetic field. Accumulation of Ca, Mg and P for different exposure (A: 10 mT, B: 50 mT, C: 100 mT) and durations (30, 60, 180, 180, 240, 360 min). Means ± SD, n = 7.
Fig. 2: Micro elements accumulation affected by static magnetic field. Accumulation of Mn, Fe and Zn for different exposure (A: 10 mT, B: 50 mT, C: 100 mT) and durations (30, 60, 180,
180, 240, 360 min). Means ± SD, n = 7.
Fig. 3: Sodium and potassium pump accumulation affected by static magnetic field. Accumulation of K and Na for different exposure (A: 10 mT, B: 50 mT, C: 100 mT) and durations (30, 60, 180, 180, 240, 360 min). Means ± SD, n = 7.
In the present study, ions content increased significantly with time extending analogues to Wojcik . study who found that MF increased ions if time of exposure was longer. Levels of calcium increased following exposure to SMF. In fact, it has been reported that changes in electrical conductivity of CaCl2 solution are caused by exposure to static magnetic fields. Being a second messenger, Ca is involved in regulation at all stages of plant growth and development, including growth and differentiation, photo morphogenesis and embryogenesis, the self- incompatibility responses in pollen-pistil interactions and movement of stomatal cells. Cytochemical studies indicate that cells of plant roots exposed to weak magnetic field show calcium over-saturation in all organelles and in cytoplasm unlike the control ones. Magnetic fields could enhance release of free radicals . and cause stress whereas calcium ions participate in many plant growth processes and responses to stress . thus explained Ca high average. showed that a
static MF exerts the strong and reproducible effect of reducing apoptosis in several cell systems. This effect is mediated by the MF’s ability to increase Ca influxes. Moreover, Mg, K , Fe, Mn, Zn and Na were also affected under SMF and increased significantly while P decreased with raising intensity and time of exposure. Analogues with Esitken and Turan. study which indicated that increasing MF strength from control to 0.384 T increased contents of N, K, Ca, Mg, Fe, Mn, Na and Zn but reduced P and S content the leaves of strawberry. In addition, results may vary according to plant organs, Wojcik. study indicated that MF increased contents of (Mg, Fe and Cu) in buckwheat (Hruszowska sp.) grain and (P, Ca, K and Zn) in straw.
In conclusion, ions accumulation was affected by magnetic field in date palm plants. Magnetic field may play an important role in cations uptake capacity and has a positive effect on immobile plant nutrient uptake which raise the products nutrition value of date palm. The SMF treatment could be a promising technique for agricultural improvements but extensive research is still required.
The authors would like to thank Dr. Saud Al- Fattah at Reserves Assessment and Development Studies Division of Saudi Aramco for reviewing the manuscript and the use of analytical facilities.
- Goodman, E.M., B. Greenebaum and M.T. Marron, 1995. Effects of electromagnetic fields on molecules and cells. Int. Rev. Cyto1., 158: 279- 338.
- Belyavskaya, N.A., 2004. Biological effects due to weak magnetic field on plants. Adv. Space Res., 34: 1566-1574.
- Johnson, C.C. and A.W. Guy, 1972. Non-ionizing electrostatic wave effects in biological materials and systems. Proc. IEEE, 60: 692-718.
- Kindzelskii, A.L. and H.R. Petty, 1997. Extremely low frequency electric fields promote metabolic resonance and cell extension during neutrophil migration. J. Allergy Clin. Immunol., 99: S317.
- Goodman, R. and M. Blank, 2002. Insights into Electromagnetic Interaction Mechanisms. J. Cellular Physiol., 192: 16-22.
- Belyavskaya, N.A., V.M. Fomicheva, R.D. Govorun and V.I. Danilov, 1992. Structural- functional organization of the meristem cells of pea, lentil and flax roots in conditions of screening the geomagnetic field. Biophysics, 37: 657-666.
- Xiyao, B., M. Ancheng, M. Jingrun, L. Xiaoling, Y. Li and W. Qingzhao, 1988. Physiological and biochemical experiments in electrostatically treated seeds. Proceedings of international conference on modern electrostatics, Beijing, China, 161-165.
- Morar, R., R. Munteanu, E. Simion, I. Munteanu, 1999. Electrostatic treatment of bean seeds. IEEE- IA, 35: 208-212.
- Moon, J. and H. Chung, 2000. Acceleration of germination of tomato seeds by applying AC electric and magnetic fields. J. Electrostatics, 48: 103-114.
- Aladjadjiyan, A. and T. Ylieva, 2003. Influence of stationary magnetic field on early stages of the development of tobacco seeds (Nicotina tabacum). J.C.E.A., 4: 132-138.
- Sharaf El-Deen, S., 2003. Improvement of some characters of edible mushroom with magnetic field. Bull NRC Egypt, 28: 709-717.
- Yao, Y., Y. Li, Y. Yang and C. Li, 2005. Effect of seed pretreatment by magnetic field on the sensitivity of cucumber (Cucumis sativus) seedlings to ultraviolet-B radiation. Env. Exp. Botany, 54: 286-294.
- Pietruszewski, S., 1999. Influence of pre-sowing magnetic biostimulation on germination and yield of wheat. Int. Agrophysics, 13: 241-244.
- Pietruszewski, S. and S. Wójcik, 2000. Effect of magnetic field on yield and chemical composition of sugar beet roots. Int. Agrophysics, 14: 89-92.
- Esitken, A. and M. Turan, 2003. Alternating magnetic field effects on yield and plant nutrient element composition of strawberry (Fragaria x ananassa cv. Camarosa). Acta Agric. Scand. Sect. B. Soil and Plant Sci., 54: 135-139.
- Wojcik, S., 1995. Effect of the pre-sowing magnetic biostimulation of the buckwheat seeds on the yield and chemical composition of buckwheat grain. Cur. Adv. Buckwheat Res., 93: 667-674.
- Zarcinas, B.A., B. Cartwright and L.R. Spouncer, 1987. Nitric acid digestion and multielement analysis of plant material by inductively coupled plasma spectrometry. Comm. Soil Sci. Plant Anal., 18: 131-146.
- Ryan, A., 2005. Rapid measurement of major, minor and trace elements in plant and food material using the Varian 730-ES ICP-OES a p p l i c a t i o n n o t e n u m b e r 3 3 . http://www.varianic.com.
- Soltani, F., A. Kashi and M. Arghavani, 2006. Effect of magnetic field on Ocimum basilicum seed germination and seedling growth. International symposium on the labiatae: advances in production, biotechnology and utilisation. ISHS Acta Horticulturae, 723.
- Liboff, A.R., 1997. Electric-field ion cyclotron resonance. Bioelectromag., 18: 85-7.
- Kinouchi, Y., S. Tanimoto, T. Ushita, K. Sato, H. Yamaguchi and H. Miyamoto, 1988. Effects of static magnetic fields on diffusion in solutions. Bio-electromagnetics, 9: 159-166.
- Van Dijk, B., J.K.H. Carpenter, A.J. Hoff and P.J. Hore, 1998. Magnetic field effects on the recombination kinetics of radical pairs. J. Phys. Chem., 102: 464-47.
- Kurinobu, S. and Y. Okazaki, 1995. Dielectric constant and conductivity of one seed in the germination process. Annual Conference Record of IEEE/IAS, 1329-1334.
- Stange, B.C., R.E. Rowland, B.I. Rapley and J.V. Podd, 2002. ELF Magnetic field increase amino acid uptake into Vicia faba L. roots and alter ion movement across the plasma membrane. Bioelectromag., 33: 347-354.
- Marschner, H., 1995.Mineral Nutrition of Higher Plants 2nd edition. Academic Press, London.
- Ayrapetyan, S.N., K.V. Grigorian, A.S. Avanesian, and K.V. Stamboltsian, 1994. Magnetic fields alter electrical properties of solutions and their physiological effects. Bioelectromag.,15: 133-142.
- Medvedev, S.S., 2005. Calcium signaling system in plants. Russian J Plant Physiol, 52: 249-270.
- Parola, A., K. Kost, G. Katsir, E. Monselise, and R. Cohen-Luria, 2006. Radical scavengers suppress low frequency EM F enhanced proliferation in cultured cells and stress effects in higher plants. The Environmentalist, 25: 103-111.
- Trewavas, A.J. and R. Malho, 1998. Ca2+ signaling in plant cells: the big network! Cur. Opin. Plant Biol., 1: 428-433.
- Fanelli, C., S. Coppola, R. Barone, C. Colussi, G. Gualandi, P. Volge and L. Ghibelli, 1999. Magnetic fields increase cell survival by inhibiting
25. Duarte Diaz, C.E., J.A. Riquenes, B. Sotolongo, M.A. Portuondo, E.O. Quintana and R. Perez, 1997. Effects of magnetic treatment of irrigation water on the tomato crop. [abstract]. Hort. Abst., 69: 494.
apoptosis via modulation of Ca2+
FASEB J., 13: 95-102.